Commission Delegated Regulation (EU) 2019/2035 of 28 June 2019 supplementing Regulation (EU) 2016/429 of the European Parliament and of the Council as regards rules for establishments keeping terrestrial animals and hatcheries, and the traceability of certain kept terrestrial animals and hatching eggs (Text with EEA relevance)

• 2.5. Sampling frame and frequency of sampling:
• (a) in the establishment keeping poultry:
• (i) sampling for Salmonella Pullorum and Salmonella Gallinarum: Species Time of sampling Number of birds to be sampled/Number of environmental samples Breeding poultry Productive poultry Gallus gallus, Meleagris gallopavo, Numida meleagris, Coturnix coturnix, Phasianus colchicus, Perdix perdix and Anas spp At the point of lay During production at least once a year 60
• (ii) sampling for Salmonella arizonae: Species Time of sampling Number of birds to be sampled/Number of environmental samples Breeding poultry Productive poultry Meleagris gallopavo At the point of lay During production at least once a year 60
• (iii) the number of birds to be sampled in accordance with points (i) and (ii) may be adapted by the competent authority to the known prevalence of infection in the specific Member State concerned and its past incidence in the establishment. In any case a statistically valid number of samples for serological/bacteriological testing to detect infection shall always be taken;
• (b) in the hatchery, samples shall be collected and examined at least once every 6 weeks. The testing shall include at least:
• (i) one pooled sample of down and meconium from chicks from each hatcher; and
• (ii) sample of:
• — either 10 second grade chickens and 10 dead-in-shell chickens from every flock of origin present in a hatcher on the day of sample collection, or
• — 20 second grade chickens from every flock of origin present in a hatcher on the day of sample collection.

• 2.6. Processing of samples and testing methods:
• (a) samples collected must be subject to:
• (i) serological testing (⁶);
• (ii) bacteriological testing either as an alternative or in addition to serological testing referred to in point (i); however, samples for bacteriological testing must not be taken from poultry or eggs that have been treated with antimicrobial medicinal products during the two to three weeks prior to testing;
• (b) samples collected must be processed as follows:
• (i) direct enrichment in Selenite-cysteine broth for faecal/meconium and intestinal samples or other appropriate media suitable for samples where competing flora is expected;
• (ii) non-selective pre-enrichment followed by selective enrichment in soya based Rappaport-Vassiliadis (RVS) broth or Müller-Kauffmann Tetrathionate-Novobiocin broth (MKTTn) for samples (such as embryos dead-in-shell) where competing flora is expected to be minimal;
• (iii) direct plating of aseptically collected tissues on to a minimally selective agar, such as MacConkey agar;
• (iv) Salmonella Pullorum and Salmonella Gallinarum do not readily grow in the modified semi-solid Rappaport Vassiliadis (MSRV) medium that is used for monitoring of zoonotic Salmonella spp. in the Union, but it is suitable for Salmonella arizonae;
• (v) detection techniques must be capable of differentiating serological responses to Salmonella Pullorum and Salmonella Gallinarum infection from serological responses due to the use of Salmonella Enteritidis vaccine, where this vaccine is used (⁷). Such vaccination must therefore not be used if serological monitoring is to be used. If vaccination has been used, bacteriological testing must be used, but the confirmation method used must be capable of differentiating live vaccinal strains from field strains.

• 2.7. Results: A flock is considered positive when, following the positive results of the testing performed in accordance with points 2.3 to 2.6, a second test of an appropriate type confirms the infection by the disease agents.

• 3.Diseases surveillance for Mycoplasma spp. of relevance for poultry:

• 3.1. Identification of infection with the following agents:
• (a) Mycoplasma gallisepticum;
• (b) Mycoplasma meleagridis.

• 3.2. Target species:
• (a) Mycoplasma gallisepticum: Gallus gallus, Meleagris gallopavo;
• (b) Mycoplasma meleagridis: Meleagris gallopavo.

• 3.3. Examinations: Each flock must be clinically examined during each laying or productive period at the best time for detecting the disease in question.

• 3.4. Sampling matrix: Samples to be taken from each flock in the establishment keeping poultry, as appropriate:
• (a) blood;
• (b) sperm;
• (c) swabs taken from the trachea, the choanae or the cloaca;
• (d) post mortem tissues, especially air sacs from day-old chicks with lesions;
• (e) in particular for the detection of Mycoplasma meleagridis, oviduct and penis of turkeys.

• 3.5. Sampling frame and frequency of sampling:
• (a) sampling for Mycoplasma gallisepticum: Species Time of sampling Number of birds to be sampled Breeding poultry Productive poultry Gallus gallus at 16 weeks of age at the point of lay and then every 90 days During production every 90 days 60 60 60 Meleagris gallopavo at 20 weeks of age at the point of lay and then every 90 days During production every 90 days 60 60 60
• (b) sampling for Mycoplasma meleagridis: Species Time of sampling Number of birds to be sampled Breeding poultry Productive poultry Meleagris gallopavo at 20 weeks of age at the point of lay and then every 90 days During production every 90 days 60 60 60
• (c) the number of birds to be sampled according to points (a) and (b) may be adapted by the competent authority to the known prevalence of infection in the specific Member State concerned and its past incidence in the establishment. In any case a statistically valid numbers of samples for serological/bacteriological testing shall always be taken.

• 3.6. Examinations, sampling and testing methods: Testing for the presence of infection by serological, bacteriological and molecular tests must be carried out by validated methods recognised by the competent authority.

• 3.7. Results: A flock is considered positive when, following the positive results of the testing performed in accordance with points 3.3 to 3.6, a second test of an appropriate type confirms the infection by the disease agents.

PART 3

Additional information on diagnostic techniques

Laboratories that have been designated by the competent authority to carry out the testing as required in Parts 1 and 2 of this Annex may consult the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organisation for Animal Health (OIE), Edition 2018 for further detailed description of the diagnostic techniques.

ANNEX III

MEANS OF IDENTIFICATIONS OF KEPT TERRESTRIAL ANIMALS

The means of identification of kept terrestrial animals are as follows:

• (a) conventional ear tag;

• (b) conventional pastern band;

• (c) electronic ear tag;

• (d) ruminal bolus;

• (e) injectable transponder;

• (f) electronic pastern band;

• (g) tattoo;

• (h) leg-ring.

ANNEX IV

TECHNICAL REQUIREMENTS FOR TRANSPONDERS USED FOR THE IDENTIFICATION OF DOGS, CATS AND FERRETS

Transponders used to individually identify dogs, cats and ferrets shall:

• (a) comply with the code structure and technical specifications of radio frequency identification as defined in ISO standards 11784 and 11785;

• (b) be evaluated in accordance with ISO standard 24631-1 to ensure compliance with ISO standards 11784 and 11785.

ANNEX V

INFORMATION TO BE CONTAINED IN THE PASSPORT FOR DOGS, CATS AND FERRETS

Passports for dogs, cats and ferrets shall contain the following information:

• (a) the location of the transponder, or where applicable the tattoo;

• (b) the date of application, or where applicable the date of reading of the transponder or the tattoo;

• (c) the individual identification code displayed by the transponder, or where applicable the tattoo;

• (d) the name and date of birth as stated by the operator, or where applicable the pet owner;

• (e) the species, breed, sex, colour, and any notable or discernible features or characteristics of the pet animal;

• (f) the name and contact information of the operator, or where applicable the pet owner;

• (g) the name, contact information and signature of the veterinarian issuing or completing the passport;

• (h) the signature of the operator, or where applicable the pet owner;

• (i) the date of issuing of the passport;

• (j) details of the anti-rabies vaccination;

• (k) the date of blood sampling for the rabies antibody titration test;

• (l) details of the treatment against infestation with Echinococcus multilocularis;

• (m) compliance with any risk-mitigating measures for diseases other than infection with rabies virus and infestation with Echinococcus multilocularis;

• (n) other relevant information regarding the health status of the pet animal.