The Animal By-Products Order 1999
Made: 8th March 1999
Coming into force: 1st April 1999
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PART I — INTRODUCTION
Title and commencement
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Extension of definitions of “animals” and “poultry”
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Interpretation and scope
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PART II — DISPOSAL OF HIGH RISK AND LOW RISK MATERIAL
Scope of Part II
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Restrictions on disposal of animal by-products
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Collection and transport of animal by-products
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Approval of premises and equipment for rendering animal by-products
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Operation of approved rendering plants
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Sampling the rendered product
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Incineration
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Burial of animal by-products
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Petfood, pharmaceutical and technical premises
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Registration of premises used for the feeding of animal by-products to zoo, circus or fur animals, recognised packs of hounds or maggots farmed for fishing bait
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Approval of knackers' yards
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Operation of knackers' yards and supply of feedingstuffs from knackers' yards
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Approval and operation of laboratories
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Records for animal by-products
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Records for approved laboratories
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PART III — CATERING WASTE INTENDED FOR FEEDING TO PIGS AND POULTRY
Feeding catering waste to ruminants, pigs and poultry
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Catering waste from a means of transport from outside Great Britain
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Transporting unprocessed catering waste
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Approval of premises producing swill from catering waste
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Operation of premises approved to process catering waste
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Records for premises approved to process catering waste
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PART IV — SWILL FOR USE AS FEEDINGSTUFFS
Consigning swill
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Feeding swill to pigs or poultry
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PART V — GENERAL
Notice requiring the disposal of animal by-products or catering waste
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Cleansing and disinfection
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Powers of inspectors
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Form of approvals, etc.
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Compliance with notices
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Form of records
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Enforcement
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Transitional provisions
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Revocations and consequential amendments
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SCHEDULE 1 — REQUIREMENTS FOR RENDERING PLANTS
General requirements
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Clean and unclean areas
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Cleansing and disinfection facilities
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Equipment
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Laboratories
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SCHEDULE 2 — RENDERING
PART I — RENDERING STANDARDS
Mammalian animal by-products
1
- (1) An operator shall render mammalian animal by-products in accordance with either—
- (a) Method 1 of Part II of this Schedule; or
- (b) any of the other methods in Part II of this Schedule if, after rendering, the resulting proteinaceous material is disposed of by burial, incineration, or a similar method which ensures that it will not enter any food or feed chain and will not be used as fertiliser.
- (2) This paragraph shall not apply in relation to the rendering of the following mammalian material—
- (a) low risk material for the production of petfood;
- (b) hides, skins, hooves, horns or hair;
- (c) blood or blood products;
- (d) milk or milk products; or
- (e) glands, tissues or organs for pharmaceutical use.
High risk material
2
An operator shall render non-mammalian high risk material, and mammalian high risk material specified in paragraph 1(2) of this Schedule, in accordance with either—
- (a) Method 1 of Part II of this Schedule; or
- (b) any of the other methods in Part II of this Schedule (either in accordance with the parameters for the selected method set out in the Schedule or in accordance with different parameters set out in the approval) provided that the rendered material complies with the microbiological standards in article 3(2).
Low-risk material
3
An operator shall render non-mammalian low risk material, and mammalian low risk material specified in paragraph 1(2) of this Schedule, in accordance with either—
- (a) paragraph 2 above; or
- (b) a method and parameters specified in the approval which ensure that the rendered material complies with the microbiological standards in article 3(2).
Part-rendering
4
Animal by-products may be part-rendered by any method approved by the appropriate Minister if, after part-rendering, the material is disposed of in accordance with article 5.
Non-mammalian animal by-products used for the production of swill
5
Notwithstanding the requirements of paragraphs 2 or 3 above, in the case of non-mammalian high risk or low risk material which is being rendered for the production of swill for feeding to pigs or poultry, the material shall be rendered for at least 60 minutes at a temperature of not less than 100°C or by an alternative method specified in the approval.
Gelatin and rendered fats
6
The preceding methods and parameters shall not apply in relation to the rendering of animal by-products for the production of gelatin or rendered fats. Any animal by-products other than gelatin or rendered fats remaining after production shall be disposed of in accordance with article 5.
Hides
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Hides shall be either rendered in accordance with the preceding provisions of this Schedule or salted using sodium chloride.
Re-rendering material
8
If the required parameters are not achieved during any rendering operation, the material shall be rendered again so that those parameters are achieved.
PART II — RENDERING METHODS
METHOD 1 — CONTINUOUS OR BATCH PRESSURE
Reduction
Time, temperature and pressure
METHOD 2 — NATURAL FAT BATCH
Reduction
Time and temperature
METHOD 3 — NATURAL FAT
Reduction
Time and temperature
METHOD 4 — ADDED FAT
Reduction
Time and temperature
METHOD 5 — DEFATTED
Reduction
Time and temperature
METHOD 6 — AQUATIC ANIMALS
SCHEDULE 3 — SAMPLING AND TESTING METHODS
PART I — MANNER OF SAMPLING
METHOD 1
1
In accordance with the following table, samples of approximately equal size shall be extracted evenly from the whole of the rendered material. These samples shall then be divided into groups of approximately equal numbers, the number of groups being the number of aggregate samples specified in the table. The samples in each group shall then be mixed together to form aggregate samples.
| Total quantity of rendered material consigned from the premises | Number of samples extracted | Number of aggregate samples obtained by mixing the relevant number of samples |
|---|---|---|
| Loose animal protein | ||
| up to 1 tonne | 7 | 1 |
| 1—2.5 tonne | 7 | 2 |
| 2.5—10 tonnes | √20×weight of sampled portion in tonnes | 2 |
| 10—40 tonnes | √20×weight of sampled portion in tonnes | 3 |
| over 40 tonnes | √20×weight of sampled portion in tonnes | 4 |
| (maximum—40 incremental samples) | ||
| Bagged animal protein | ||
| 1—16 bags | 4 | 1 |
| 17—200 bags | √number of bags of sampled portion | 2 |
| 201—800 bags | √number of bags of sampled portion | 3 |
| over 800 bags | √number of bags of sampled portion | 4 |
| (maximum—40 incremental samples) |
2
Each aggregate sample shall be placed into a separate sterile receptacle and each shall be thoroughly mixed by stirring or shaking.
3
Approximately equal amounts shall be taken from each aggregate sample and mixed so as to provide a single final sample of approximately 500 grams. This final sample shall be transferred into a suitable sterile screw top container which shall then be sealed and marked to indicate its identity.
METHOD 2
3
Approximately equal amounts shall be taken from each aggregate sample and mixed so as to provide a single final sample of approximately 500 grams. This final sample shall be transferred into a suitable sterile screw top container which shall then be sealed and marked to indicate its identity.
PART II — METHOD FOR THE ISOLATION OF CLOSTRIDIUM PERFRINGENS
Time of testing
Samples
Inoculations
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Once the agar has set, each agar plate shall be overlaid with a further 10 ml SF agar at a temperature of 49°C±1°C. Once the overlay has set and with the plate lids uppermost the plates shall be incubated anaerobically at 37°C±1°C for 20 hours±2 hours.
Samples with colonies of Clostridium perfringens
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After incubation each set of duplicate plates shall be examined for colonies characteristic of Clostridium perfringens(black). The sample provisionally fails if any colonies characteristic of Clostridium perfringensare present, in which case the following procedure shall be followed to establish whether or not the colonies are Clostridium perfringens.
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In the case of each plate, 10 characteristic colonies of Clostridium perfrin gensshall be subcultured on to a further SF agar plate. If there are less than 10 colonies on the plate, all characteristic colonies shall be subcultured on to the further plate. The plates shall be incubated anaerobically at 37°C±1°C for 20 hours±2 hours.
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If the surface area of the plates is overgrown and it is not possible to select well isolated characteristic colonies, 10 suspect colonies shall be subcultured on to duplicate SF agar plates and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.
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One characteristic colony from each plate shall be subcultured on to SF agar and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.
Subcultured colonies
9
After incubation each plate shall be examined for colonies characteristic of Clostridium perfringens. All colonies characteristic of Clostridium perfrin gensshall be—
- (a) stab inoculated into motility nitrate medium[^f00018]; and
- (b) inoculated into either lactose gelatin medium[^f00019] or charcoal gelatin discs[^f00020];
and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.
Examination of subcultures
Motility
10
The motility nitrate medium shall be examined for the type of growth along the stab line. If there is evidence of diffuse growth out into the medium away from the stab line, the bacteria shall be considered to be motile.
Reduction of nitrate to nitrite
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After examination of the motility nitrate medium, 0.2 ml to 0.5 ml of nitrite detection reagent shall be added to it. The formation of a red colour confirms that the bacteria have reduced nitrate to nitrite. Cultures that show a faint reaction (i.e. a pink colour) should be discounted. If no red colour is formed within 15 minutes, a small amount of zinc dust shall be added and the plate allowed to stand for 15 minutes. If a red colour is formed after the addition of zinc dust no reduction of nitrate to nitrite has taken place.
Production of gas and acid from lactose and liquefaction of gelatin
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The lactose gelatin medium shall be examined for the presence of small gas bubbles in the medium.
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The lactose gelatin medium shall be examined for colour. A yellow colour indicates fermentation of lactose.
14
The lactose gelatin medium shall be chilled for one hour at 2—8°C and then checked to see if the gelatin has liquefied. If the medium has solidified it shall be re-incubated anaerobically for a further 18—24 hours, the medium chilled for a further one hour at 2—8°C and again checked to see if the gelatin has liquefied.
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The presence of Clostridium perfringensshall be determined on the basis of the results from paragraphs 10 to 14. Bacteria which produce black colonies on SF agar, are non-motile, reduce nitrate to nitrite, produce gas and acid from lactose and liquefy gelatin within 48 hours shall be considered to be Clostridium perfringens.
Control Tests
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Control tests shall be carried out each day that a test is initiated using
- (a) Clostridium perfringensno more than seven days old at the time of use;
- (b) Escherichia coliNCTC 10418[^f00021] or equivalent not more than seven days old at the time of use; and
- (c) rendered animal protein which is free of Clostridium perfringens.
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10 gram portions of the rendered animal protein shall be placed aseptically in each of two jars containing 90 ml Buffered Peprone Water (BPW)[^f00022] and mixed thoroughly until the samples are evenly suspended.
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One colony of Clostridium perfringensshall be placed in 10 ml BPW and mixed to form an even suspension. 0.1 ml of the suspension shall be added to the suspension in the preceding paragraph. This shall be repeated for Escherichia coli.
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These are then treated and examined in the same way as test samples. If no typical colonies are formed then that day’s testing shall be invalid and shall be repeated.
PART III — METHODS FOR THE ISOLATION OF SALMONELLA
A. BACTERIOLOGICAL METHOD
Day 1
Day 2
Day 3
Day 4
Day 5
B. ELECTRICAL CONDUCTANCE METHOD
Day 1
Day 2
Day 3
Day 4
Day 5
PART IV — METHOD FOR THE ISOLATION OF ENTEROBACTERIACEAE
Samples
Inoculations
Samples with colonies of Enterobacteriaceae
Examination of subcultures
Controls
SCHEDULE 4 — REQUIREMENTS FOR KNACKERS' YARDS
General requirements
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Clean and unclean areas in premises producing feedingstuffs for animals whose flesh is not intended for human consumption
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Reception and storage facilities in premises not producing feedingstuffs
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Hides
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Cleansing and disinfection facilities
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Repair of installations
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Products of knackers' yards
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Feedingstuffs
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SCHEDULE 5 — REQUIREMENTS FOR PREMISES PROCESSING CATERING WASTE
General requirements
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Clean and unclean areas
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Processing standards
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Cleansing and disinfection facilities
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Equipment
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SCHEDULE 6 — REVOCATIONS AND CONSEQUENTIAL AMENDMENTS
PART I — REVOCATIONS
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PART II — AMENDMENTS
The Bovine Spongiform Encephalopathy (No. 2) Order 1996
1
In article 4(1) of the Bovine Spongiform Encephalopathy (No. 2) Order 1996 (Interpretation) for the definition of “rendering” there shall be substituted—
- “rendering” means treating material at a rendering, fishmeal or other plant in accordance with Schedule 2 to the Animal By-Products Order 1999;
Signed
Jeff Rooker — Minister of State Ministry of Agriculture, Fisheries and Food — 1999-03-03
Sewel — Under Secretary of State Scottish Office — 1999-03-02
Signed by authority of the Secretary of State for Wales
Jon Owen Jones — Parliamentary Under Secretary of State Welsh Office — 1999-03-08
Explanatory note
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Footnotes
[^f00001]: 1981 c. 22. See section 86(1)(c) for a definition of “the Ministers”.
[^f00002]: S.I. 1997/2965.
[^f00003]: S.I. 1997/2964.
[^f00004]: OJ No. L 378, 31.12.82, as amended by Council Regulation (EEC) No. 3768/85 (OJ No. L 362, 31.12.85, p. 8), Commission Decision 89/162/EEC (OJ No. L 61, 4.3.89, p. 48) and Commission Decision 92/450/EEC (OJ No. L 248, 28.8.92, p. 77).
[^f00005]: Published by the British Standards Institute, British Standards House, 389 Chiswick High Road, London W4 4AL.
[^f00006]: Published by the British Standards Institute; see above.
[^f00007]: Published by the British Standards Institute; see above.
[^f00008]: Published by the Nordic Committee on Food Analysis, National Veterinary Institute, Department of Food and Hygiene, PO Box 8156, N-0033, Oslo, Norway.
[^f00009]: Published by the British Standards Institute; see above.
[^f00010]: S.I. 1995/539 as amended by S.I. 1995/731, S.I. 1995/1763, S.I. 1995/2200, S.I. 1995/2418, S.I. 1995/3124, S.I. 1995/3189, S.I. 1996/1148, S.I. 1996/2235, S.I. 1997/1729 and S.I. 1997/2074.
[^f00011]: S.I. 1995/540 as amended by S.I. 1995/1763, S.I. 1995/2200, S.I. 1995/3205 and S.I. 1997/1729.
[^f00012]: S.I. 1995/2148 as amended by S.I. 1995/3205.
[^f00013]: S.I. 1994/3082 as amended by S.I. 1995/1763, S.I. 1995/2200, S.I. 1995/3205 and S.I. 1996/1499.
[^f00014]: S.I. 1995/3205 as amended by S.I. 1996/3124.
[^f00015]: S.I. 1992/3303 as amended by S.I. 1996/827 and S.I. 1997/2894.
[^f00016]: S.I. 1973/1936 as amended by S.I. 1987/232 and S.I. 1996/826.
[^f00017]: Shahidi-Ferguson agar—See Shahidi, S. A. and Ferguson, A. R. (1971) Applied Microbiology 21:500—506. American Society for Microbiology, 1913 1 St N.W., Washington DC 20006, USA.
[^f00018]: Motility nitrate medium—See Hauschild AHW, Gilbert RJ, Harmon SM, O'Keefe MF, Vahlefeld R, (1997) ICMSF Methods Study VIII, Canadian Journal of Microbiology 23, 884—892. National Research Council of Canada, Ottawa ON K1A 0R6, Canada.
[^f00019]: Lactose gelatin medium—See Hauschild AHW, Gilbert RJ, Harmon SM, O'Keefe MF, Vahlefeld R, (1997) ICMSF Methods Study VIII, Canadian Journal of Microbiology 23, 884—892.
[^f00020]: Charcoal gelatin discs—See Mackie and McCartnay, (1996) Practical Medical Microbiology 14, 509. Churchill Livingstone, Robert Stevenson House, 1—3 Baxter’s Place, Leith Walk, Edinburgh EH1 3AF.
[^f00021]: The National Collection of Type Cultures, Central Public Health Laboratory, 61 Colindale Ave, London NW9 5HT.
[^f00022]: Buffered Peptone Water—See Edel, W. and Kampelmacher, E. H. (1973) Bulletin of World Health Organisation, 48: 167—174, World Health Organisation Distribution and Sales, CH-1211, Geneva 27, Switzerland (ISSN 0042—9686).
[^f00023]: Rappaports Vassiliadis Broth—See Vassiliadis, P., Pateraki, E., Papaiconomou, N., Papadakis, J. A., and Trichopoulos, D. (1976) Annales de Microbiologie (Institut Pasteur) 127B: 195—200. Elsevier, 23 rue Linois, 75724 Paris, Cedex 15, France.
[^f00024]: Brilliant Green Agar—See Edel, W. and Kampelmacher, E. H. (1969) Bulletin of World Health Organisation, 41:297—306, World Health Organisation Distribution and Sales, CH-1211, Geneva 27, Switzerland (ISSN 0042—9686).
[^f00025]: Xylose Lysine Deoxycholate Agar—See Taylor, W. I. (1965) American Journal of Clinical Pathology, 44:471—475, Lippincott and Raven, 227 E. Washington Street, Philadelphia PA19106, USA.
[^f00026]: MacConkey agar—See (1963) International Standards for Drinking Water. World Health Organisation Distribution and Sales, CH-1211, Geneva 27, Switzerland.
[^f00027]: Buffered Peptone Water/Lysine/Glucose—See Ogden, I. D. (1988) International Journal of Food Microbiology 7:287—297, Elsevier Science BV, P.O. Box 211, 1000 AE, Amsterdam, Netherlands (ISSN 0168—1695).
[^f00028]: Selenite Cystine Trimethylamine-N-oxide Dulcitol—See Easter, M. C. and Gibson, D. M., (1985) Journal of Hygiene 94:245—262, Cambridge University Press, Cambridge.
[^f00029]: LysineDecarboxylase Glucose—See Ogden, I. D., (1988) International Journal of Food Microbiology 7: 287—297, Elsevier Science BV, P.O. Box 211, 1000 AE, Amsterdam, Netherlands (ISSN 0168—1695).
[^f00030]: Violet Red Bile Glucose Agar—See Mossel, D. A. A., Eelderink, I., Koopmans, M., van Rossem, F. (1978) Laboratory Practice 27 No. 12 1049—1050; Emap Maclaren, PO Box 109, Maclaren House, 19 Scarbrook Road, Croydon CR9 1QH.
[^f00031]: An arithmetic mean of 30 is equivalent to 3×10² colony forming units per gram of original sample.
[^f00032]: S.I. 1973/1936.
[^f00033]: S.I. 1987/232.
[^f00034]: S.I. 1996/826.
[^f00035]: S.I. 1989/661.
[^f00036]: S.I. 1992/3303.
[^f00037]: S.I. 1996/827.
[^f00038]: S.I. 1997/2894.
[^f00039]: S.I. 1996/3183 to which there are amendments not relevant to this Order.
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Editorial notes
[^key-3006468e4111128bbb461cef1ebeff4c]: Order revoked: (E.) (1.7.2003) by The Animal By-Products Regulations 2003 (S.I. 2003/1482), regs. 1, 51(2); (S.) (1.10.2003) by The Animal By-Products (Scotland) Regulations 2003 (S.S.I. 2003/411), reg. 1(1), sch. 6 (with reg. 50, sch. 4); and (W.) (31.10.2003) by The Animal By-Products (Wales) Regulations 2003 (S.I. 2003/2756), regs. 1, 51(2)
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