The Animal By-Products (Wales) Regulations 2003
Made: 29th October 2003
Coming into force: 31st October 2003
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PART 1 — Introduction
Title, application and commencement
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Interpretation
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Approvals etc.
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PART 2 — Collection, transportation, storage, handling, processing and disposal of animal by-products
Category 1 material
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Category 2 material
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Category 3 material
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Mixing mammalian and non-mammalian by-products
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Collection, transportation and storage
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PART 3 — Restrictions on access to animal by-products and their use
Access to animal by-products
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Restrictions on use
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Pasture land
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Intra-species recycling of fish
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PART 4 — Approved premises and competent authorities
The competent authority
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Approval of premises
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Biogas and composting plants
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Composting catering waste on the premises on which it originates
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Processing and intermediate plants own checks
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Sampling at processing plants
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Sampling at biogas and composting plants
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Samples sent to laboratories
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Laboratories
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PART 5 — Placing animal by-products and processed products on the market
Placing on the market of processed animal protein and other processed products that could be used as feed material
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Placing on the market of petfood, dogchews and technical products
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Placing on the market of compost or digestion residues for use on agricultural land
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PART 6 — Derogations
Competent authority for Chapter V of the Community Regulation
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Derogations regarding the use of animal by products
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Collection centres
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Burial of pet animals
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Burial in the event of a disease outbreak
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Burning and burial of bees and apiculture products
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PART 7 — Records
Records
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Records for consigning, transporting or receiving animal by-products
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Records for burying or burning of animal by-products
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Records for disposal or use on premises
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Delivery records to be kept by operators of approved biogas and composting plants
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Treatment records for biogas and composting plants
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Records for approved laboratories
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Records to be kept for consignments of compost or digestion residue
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PART 8 — Administration and enforcement
Grants of approvals
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Suspension, amendment or revocation of approvals, authorisations and registrations
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Appeals
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Notice requiring the disposal of animal by-products or catering waste
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Cleansing and disinfection
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Compliance with notices
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Powers of entry
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Obstruction
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Penalties
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Enforcement
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Service of notices and other documents
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Transitional provisions
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Amendments and Revocations
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SCHEDULE 1 — Additional requirements for biogas and composting plants
PART 1
Premises
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- (1) There must be —
- (a) a reception area in which untreated animal by-products (including catering waste) are received,
- (b) an area in which vehicles and containers are cleansed and disinfected with adequate facilities for doing this, and
- (c) a clean area in which treated compost or digestion residue are stored.
- (2) The clean area must be, adequately separated from the reception area and the area in which vehicles and containers are cleansed and disinfected so as to prevent contamination of the treated material. Floors must be laid so that liquid cannot seep into the clean area from the other areas.
- (3) The reception area must be easy to clean and disinfect and must have an enclosed and lockable place or container to receive and store the untreated animal by-products.
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The animal by-products must be unloaded in the reception area and either —
- (a) treated immediately, or
- (b) stored in the reception area and treated without undue delay.
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The plant must be operated in such a way that —
- (a) treated material is not contaminated by untreated or partially treated material or liquids arising from it; and
- (b) partially treated material is not contaminted with material which has not been treated to the same extent or liquids arising from it.
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The operator shall identify, control and monitor suitable critical points in the operation of the plant to demonstrate that —
- (a) these Regulations and the Community Regulation are complied with;
- (b) treated material is not contaminated by untreated or partially treated material or liquids arising from it; and
- (c) partially treated material is not contaminated with material which has not been treated to the same extent or liquids arising from it.
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PART II — Treatment systems and parameters for catering waste
Composting plants
Biogas plants
SCHEDULE 2 — Testing Methods
PART I — METHOD FOR THE ISOLATION OF CLOSTRIDIUM PERFRINGENS
Time of testing
1
Tests must be begun on receipt of the sample or on the first working day which allows this method to be completed. If the test is not begun on the day of receipt the sample must be stored in a refrigerator at between 2°C and 8°C until required. If the sample has been refrigerated it must be removed from the refrigerator and stored at room temperature for at least one hour before the test is started.
Samples
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Tests must be carried out using two 10 gram portions of each sample submitted for testing. Each 10 gram sample must be placed aseptically in a sterile container containing 90 ml Clostridium perfringens diluent consisting of 0.1% peptone and 0.8% sodium chloride at a pH of 7 and mixed thoroughly until the sample is evenly suspended.
Inoculations
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For each portion of the sample 1 ml of solution must be transferred to a sterile 90 mm petri dish (in duplicate), to which 15 ml of Shahidi - Ferguson agar (SF agar)[^f00025] at a temperature of 47°C±1°C must be added and immediately gently mixed by swirling the dish with 5 clockwise and 5 anticlockwise circular movements.
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Once the agar has set, each agar plate must be overlaid with a further 10 ml SF agar at a temperature of 47°C±1°C. Once the overlay has set and with the plate lids uppermost the plates must be incubated anaerobically at 37°C±1°C for 20 hours±2 hours.
Samples with colonies of Clostridium perfringens
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After incubation each set of duplicate plates must be examined for colonies characteristic of Clostridium perfringens (black). The sample provisionally fails if any colonies characteristic of Clostridium perfringens are present, in which case the following procedure must be followed to establish whether or not the colonies are Clostridium perfringens.
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In the case of each plate, 10 characteristic colonies of Clostridium perfringens must be subcultured on to a further SF agar plate. If there are less than 10 colonies on the plate, all characteristic colonies must be subcultured on to the further plate. The plates must be incubated anaerobically at 37°C±1°C for 20 hours±2 hours.
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If the surface area of the plates is overgrown and it is not possible to select well isolated characteristic colonies, 10 suspect colonies must be subcultured on to duplicate SF agar plates and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.
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One characteristic colony from each plate must be subcultured on to SF agar and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.
Subcultured colonies
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After incubation each plate must be examined for colonies characteristic of Clostridium perfringens. All colonies characteristic of Clostridium perfringens must be —
- (a) stab inoculated into motility nitrate medium[^f00026]; and
- (b) inoculated into either lactose gelatin medium[^f00027] or charcoal gelatin discs[^f00028];
and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.
Examination of subcultures
Motility
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The motility nitrate medium must be examined for the type of growth along the stab line. If there is evidence of diffuse growth out into the medium away from the stab line, the bacteria must be considered to be motile.
Reduction of nitrate to nitrite
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After examination of the motility nitrate medium, 0.2 ml to 0.5 ml of nitrite detection reagent must be added to it. The formation of a red colour confirms that the bacteria have reduced nitrate to nitrite. Cultures that show a faint reaction (i.e. a pink colour) must be discounted. If no red colour is formed within 15 minutes, a small amount of zinc dust must be added and the plate allowed to stand for 15 minutes. If a red colour is formed after the addition of zinc dust no reduction of nitrate to nitrite has taken place.
Production of gas and acid from lactose and liquefaction of gelatin
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The lactose gelatin medium must be examined for the presence of small gas bubbles in the medium.
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Control Tests
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PART II — METHODS FOR THE ISOLATION OF SALMONELLA
A. BACTERIOLOGICAL METHOD
Day 1
Day 2
Day 3
Day 4
Day 5
B. ELECTRICAL CONDUCTANCE METHOD
1
Tests must be begun on receipt of the sample or on the first working day which allows the following method to be completed. If the test is not begun on the day of receipt the sample must be stored in a refrigerator until required. If the sample has been refrigerated it must be stored at room temperature for at least four hours before the test is started.
Day 1
2
Tests must be carried out in duplicate using two 25 gram portions of each sample submitted for testing. Each 25 gram sample must be placed aseptically in a sterile container containing 225 ml Buffered Peptone Water/Lysine/Glucose (BPW/L/G)[^f00035] and incubated at 37°C for 18 hours.
Day 2
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The incubated BPW/L/G must be added to Selenite Cystine Trimethylamine-N-Oxide Dulcitol (SC/T/D)[^f00036] and Lysine Decarboxylase Glucose (LD/G)[^f00037] media in electrical conductance cells or wells. For cells or wells containing more than 5 ml medium 0.2 ml of the BPW/L/G must be added and for cells or wells containing 5 ml or less medium 0.1 ml of the BPW/L/G must be added. Cells or wells must be connected to appropriate electrical conductance measuring equipment set to monitor and record changes in electrical conductance at 6 minute intervals over a 24 hour period. The temperature of cells and wells must be kept at 37°C.
Day 3
Day 4
5
The plates must be examined and a minimum of 3 colonies from each plate showing suspicion of Salmonella growth must be subcultured —
- (a) on to a blood agar plate;
- (b) on to a MacConkey agar plate; and
- (c) into biochemical media suitable for the identification of Salmonella.
Day 5
PART III — METHOD FOR THE ISOLATION OF ENTEROBACTERIACEAE
Samples
Inoculations
Samples with colonies of Enterobacteriaceae
Examination of subcultures
Controls
SCHEDULE 3 — Transitional Measures
PART I — INTRA-SPECIES RECYCLING BAN FOR FISH
1
In accordance with Article 1 of Commission Regulation (EC) No. 811/2003 implementing Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the intra-species recycling ban for fish, the burial and burning of animal by-products and certain transitional measures, the prohibition on the feeding of fish with processed animal protein derived from the bodies or parts of bodies of fish of the same species in Article 22(1)(a) of the Community Regulation shall not apply.
PART II — COLLECTION, TRANSPORTATION AND DISPOSAL OF FORMER FOODSTUFFS
2
For the purposes of Article 1(1) of Commission Regulation (EC) No. 813/2003, by way of derogation from Article 6(2)(f) and Article 7 of the Community Regulation, former foodstuffs which have not been mixed with any other animal by-products (other than Category 3 catering waste) may be collected, transported and disposed of or treated in the same way as catering waste.
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Where former foodstuffs are mixed with Category 1 or Category 2 material any person in possession or control of the material must ensure that it is disposed of in accordance with Article 1(2) of Commission Regulation (EC) No. 813/2003; and any person who fails to do so will be guilty of an offence.
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Where former foodstuffs are sent for disposal in an approved landfill site, any person in possession or control of the material must comply with Article 1(3) of Commission Regulation (EC) No. 813/2003 and any person who fails to do so will be guilty of an offence.
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Any person who fails to comply with any instructions given by an inspector under Article 3(3) of Commission Regulation (EC) No. 813/2003 will be guilty of an offence.
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In this Part “former foodstuffs” does not include waste from the production of products which are intended to be cooked before they are eaten.
PART III — USKED COOKING OIL IN ANIMAL FEED
Scope
Approvals
General obligations
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Collection and transportation of used cooking oil
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Approved premises and the operation of blending premises
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Approved premises
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Operators'own-checks
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Hygiene requirements in approved premises
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Specification for used cooking oil for use in animal feed
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Commercial documents
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Records
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List of premises
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PART IV — MAMMALIAN BLOOD
General
PART V — OLEOCHEMICAL PLANTS USING RENDERED FATS FROM CATEGORY 2 AND CATEGORY 3 MATERIALS
General obligations
Specific requirements
PART VI — LOW CAPACITY INCINERATION OR CO-INCINERATION PLANTS WHICH DO NOT INCINERATE OR CO-INCINERATE SPECIFIED RISK MATERIALS OR CARCASES CONTAINING THEM
General obligations
Specific requirements
SCHEDULE 4
Amendments
Amendments to the TSE (Wales) Regulations 2002
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SCHEDULE 5 — APPLICATION OF PART IV OF THE REGULATIONS TO SCHEME ANIMALS
| PROVISION OF THE REGULATIONS | EXTENT TO WHICH THE PROVISON APPLIES TO SCHEME ANIMALS |
|---|---|
| Regulation 33(3) | Not applicable |
| Regulation 33(4) | Subject to the modification that from the point at which specified risk material derived from a scheme animal is removed from the slaughterhouse, it may come into contact with any other animal material from such an animal |
| Regulation 34 | Not applicable |
| Regulation 39(3)(b) | Not applicable |
| Regulation 57 | Not applicable |
Signed
Signed on behalf of the National Assembly for Wales under section 66(1) of the Government of Wales Act 1998
D. Elis-Thomas — The Presiding Officer of the National Assembly — 29th October 2003
Explanatory note
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Footnotes
[^f00001]: S.I. 2003/1246.
[^f00002]: 1972 c. 68.
[^f00003]: OJ No. L273, 10.10.2002, p.1.
[^f00004]: OJ No. L117, 13.5.2003, p.1.
[^f00005]: OJ No. L117, 13.5.2003, p.14.
[^f00006]: OJ No. L117, 13.5.2003, p.22.
[^f00007]: OJ No. L117, 13.5.2003, p.24.
[^f00008]: OJ No. L117, 13.5.2003, p.30.
[^f00009]: OJ No. L117 13.5.2003, p.42.
[^f00010]: OJ No. L117 13.5.2003, p.44.
[^f00011]: Chapter VII was added to Annex IV by Commission Regulation (EC) No. 808/2003.
[^f00012]: These paragraphs were added by Commission Regulation (EC) No. 808/2003.
[^f00013]: Published by the British Standards Institute, British Standards House, 389 Chiswick High Road, London W4 4AL.
[^f00014]: Published by the British Standards Institute; see above.
[^f00015]: Published by the Nordic Committee on Food Analysis, National Veterinary Institute, Department of Food and Hygiene, PO Box 8156, N-0033, Oslo, Norway.
[^f00016]: Published by the British Standards Institute; see above.
[^f00017]: S.I. 1995/539 as amended by S.I. 1995/731, S.I. 1995/1763, S.I. 1995/2200, S.I. 1995/2418, S.I. 1995/3124, S.I. 1995/3189, S.I. 1996/1148, S.I. 1996/2235, S.I. 1997/1729 and S.I. 1997/2074.
[^f00018]: S.I. 1995/540 as amended by S.I. 1995/1763, S.I. 1995/2200, S.I. 1995/3205 and S.I. 1997/1729.
[^f00019]: S.I. 1995/2148 as amended by S.I. 1995/3205.
[^f00020]: S.I. 1994/3082 as amended by S.I. 1995/1763, S.I. 1995/2200, S.I. 1995/3205 and S.I. 1996/1499.
[^f00021]: S.I. 1995/3205 as amended by S.I 1996/3124.
[^f00022]: S.I. 1999/646.
[^f00023]: S.I. 2001/1735 (W.122).
[^f00024]: 1998 c. 38.
[^f00025]: Shahidi-Ferguson agar- See Shahidi, S. A. and Ferguson, A. R. (1971) Applied Microbiology 21:500-506.American Society for Microbiology, 1913 1 St N.W., Washington DC 20006, USA.
[^f00026]: Motility nitrate medium-See Hauschild AHW, Gilbert RJ, Harmon SM, O'Keefe MF, Vahlefeld R, (1997) ICMSF Methods Study VIII, Canadian Journal of Microbiology 23, 884-892. National Research Council of Canada, Ottawa ON K1A oR6, Canada.
[^f00027]: Lactose gelatin medium- See Hauschild AHW, Gilbert RJ, Harmon SM, O'Keefe MF, Vahlefield R, (1997) ICMSF Methods Study VIII, Canadian Journal of Microbiology 23, 884-892.
[^f00028]: Carcoal gelatin discs- See Mackie and McCartney, (1996) Practical Medical Microbiology 14, 509. Churchill Livingstone, Robert Stevenson House, 1-3 Baxter’s Place, Leith Walk, Edinburgh EH1 3AF.
[^f00029]: The National Collection of Type Cultures, Central Public Health Laboratory, 61 Colindale Ave, London NW9 5HT.
[^f00030]: Buffered Peptone Water — See Edel, W. and Kampelmacher, E.H. (1973) Bulletin of World Health Organisation, 48: 167-174, World Health Organisation Distribution and Sales, CH-1211, Geneva 27, Switzerland (ISSN 0042-9686).
[^f00031]: Rappaports Vassiliadis Broth — See Vassiliadis P, Pateraki E, Papaiconomou N, Papadkis J A, and Trichopoulos D (1976) Annales de Microbiologie (Institute Pasteur) 127B: 195-200, Elsevier, 23 rue Linois, 75724 Paris, Cedex 15, France.
[^f00032]: Brilliant Green Agar — See Edel W and Kampelmacher E H (1969) Bulletin of World Health Organisation 41:297-306, World Health Organisation Distribution and Sales, CH-1211, Geneva 27, Switzerland (ISSN 0042-9686).
[^f00033]: Xylose Lisene Deoxycholate Agar — See Taylor W I, (1965) American Journal of Clinical Pathology, 44:471-475, Lippincott and Raven, 227E Washington Street, Philadelphia PA 19106, USA.
[^f00034]: MacConkey agar — See (1963) International Standards for Drinking Water, World Health Distribution and Sales, CH-1211, Geneva 27, Switzerland.
[^f00035]: Buffered Peptone Water/Lysine/Glucose — See Ogden I D (1988) International Journal of Food Microbiology 7:287-297, Elsevier Science BV, PO Box 211, 1000 AE, Amsterdam, Netherlands (ISSN 0168-1695).
[^f00036]: Selenite Cystine Trimethylamine-N-Oxide Dulcitol — See Easter, M C and Gibson, D M, (1985) Journal of Hygiene 94:245-262, Cambridge University Press, Cambridge.
[^f00037]: Lysine Decarboxylase Glucose — See Ogden I D (1988) International Journal of Food Microbilogy 7:287-297, Elsevier Science BV, PO Box 211, 1000 AE, Amsterdam, Netherlands (ISSN 0168-1695).
[^f00038]: Violet Red Bile Glucose Agar — See Mossell D A A, Eelderink I, Koopmans M, van Rossem F (1978) Laboratory practice 27 No. 12 1049-1050; Emap Maclaren, PO Box 109, Maclaren House, 19 Scarbrook Road, Croydon CR9 1QH.
[^f00039]: An arithmetic mean of 30 is equivalent to 3x10² colony forming units per gram of original sample.
[^f00040]: This Part of the Schedule enforces Article 1 of Commission Regulation (EC) No. 811/2003 implementing Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the intra-species recycling ban for fish, the burial and burning of animal by-products and certain transitional measures, OJ No. L117, 13.5.2003, p.14.
[^f00041]: This Part of the Schedule enforces Commission Regulation (EC) No. 813/2003 on transitional measures under Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the collection, transport and disposal of former foodstuffs, OJ No. L117, 13.5.2003, p.22.
[^f00042]: This Part of the Schedule enforces Commission Decision 2003/320/EC on transitional measures under Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the use in feed of used cooking oils, OJ No. L117, 13.5.2003, p.24.
[^f00043]: OJ No. L 140, 30.05.2002, p.10.
[^f00044]: ICES7 polychlorinated biphenyls.
[^f00045]: This Part of the Schedule implements Commission Decision 2003/321/EC on transitional measures under Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the processing standards mammalian blood, OJ No. L117, 13.5.2003, p.30.
[^f00046]: This Part of the Schedule implements Commission Decision 2003/326/EC on transitional measures under Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the separation of Category 2 and Category 3 oleochemical plants, OJ No. L117, 13.5.2003, p.42.
[^f00047]: This Part of the Schedule implements Commission Decision 2003/326/EC on transitional measures under Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the low capacity incineration on co-incineration plants which do not incinerate or co-incinerate specified risk material or carcases containing them, OJ No. L117, 13.5.2003, p.44.
[^f00048]: S.I. 2002/1416 (W.142).
[^f00049]: OJ No. L117, 13.5.2003, p.1.
[^f00050]: OJ No. L117, 13.5.2003, p.14.
[^f00051]: OJ No. L117, 13.5.2003, p.22.
[^f00052]: OJ No. L117, 13.5.2003, p.24.
[^f00053]: OJ No. L117, 13.5.2003, p.30.
[^f00054]: OJ No. L117, 13.5.2003, p.42.
[^f00055]: OJ No. L117, 13.5.2003, p.44.
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Inoculations
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Controls
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Amendments
Editorial notes
[^key-6923fb1104eb1436024dc01d1cbb8d89]: Regulations revoked (12.5.2006) by The Animal By-Products (Wales) Regulations 2006 (S.I. 2006/1293), regs. 1, 53(2)
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